Table 3. Fluorescence quantities of the immuno-probed biofilms.a .
| MAbs | Z-position (µm) | SYTO9b | Cy3c | Cy3/SYTO9d |
| 18B6e | 0.5 | 1382.8 | 99.0 | 0.072 |
| 2.0 | 1668.6 | 127.7 | 0.077 | |
| 3.5 | 1797.4 | 365.1 | 0.203 | |
| 5.0 | 1666.0 | 797.7 | 0.479 | |
| 6.5 | 984.7 | 630.4 | 0.640 | |
| 25C11 | 0.5 | 1043.1 | 43.1 | 0.041 |
| 2.0 | 1250.7 | 107.5 | 0.086 | |
| 3.5 | 1253.3 | 356.2 | 0.284 | |
| 5.0 | 739.8 | 402.5 | 0.544 | |
| 6.5 | 218.4 | 163.3 | 0.748 | |
| 20B9 | 0.5 | 764.2 | 23.0 | 0.030 |
| 3.0 | 907.8 | 44.3 | 0.049 | |
| 5.5 | 735.8 | 165.8 | 0.225 | |
| 8.0 | 417.7 | 295.3 | 0.707 | |
| 10.5 | 151.4 | 194.1 | 1.282 | |
| Mock | 0.5 | 712.1 | 211.6 | 0.297 |
| 1.5 | 779.8 | 225.8 | 0.290 | |
| 2.5 | 803.9 | 245.7 | 0.306 | |
| 3.5 | 559.5 | 165.7 | 0.296 | |
| 4.5 | 201.1 | 51.8 | 0.258 | |
| - | 0.5 | 1040.6 | 378.5 | 0.364 |
| 1.5 | 1133.4 | 415.2 | 0.366 | |
| 2.5 | 950.4 | 345.5 | 0.364 | |
| 3.5 | 399.6 | 129.1 | 0.323 | |
| 4.5 | 128.2 | 37.6 | 0.293 |
a. The biofilm of S. epidermidis RP62A and Aap were stained by SYTO9 and Cy3, respectively. After obtaining the fluorescence photomicrographs under a CLSM (Figure 7), the fluorescence quantities of SYTO9-stained cells, Cy3-stained Aap were determined using ImageJ program.
b. “SYTO9” stands for the fluorescence quantities of SYTO9-stained cells.
c. “Cy3” stands for the fluorescence quantities of Cy3-stained Aap.
d. “Cy3/SYTO9” represents the proportion of Aap in the biofilms.
e. Fluorescence quantities of SYTO9 and Cy3 in biofilm co-cultured with MAb18B6 were obtained from other images (not shown) which captured at the area away from the boundaries of the crater-like micropores where Cy3-stained Aap was up-regulated in all Z-positions.