. 2011 Jun 7;6(6):e20918. doi: 10.1371/journal.pone.0020918

Table 5. Oligonucleotide primers used in the present study.

Primers	Sequence (5′→3′)
Primers used for PCR amplification of AapBrpt1.5^a
F	CGCGGATCCCCAGTTGATGGAGATCCGATTAC
R	CCGCTCGAGTTATTTTGTTGGACCATACTCAACAATT
Primers used for Q-PCRs in eDNA quantification^b
gyrA F	CCTTATGAAACTCGGAGATGG
gyrA R	TCAGTAGTAGTAGATTGTTGCG
serp0306 F	ATGCCACATCCACGAAAGA
serp0306 R	TGTAACTGACAATGCCCAATC
lysA F	TGACAATGGGAGGTACAAGC
lysA R	TGGTCTTCATCGTAAACAATCG
leuA F	GTGAACGGTATTGGTGAAAGAG
leuA R	GTGGTCCTTCCTTACATATAAAGC
Primers used for Q-RT-PCRs in Aap transcriptional analysis^b
gyrB F	AGAAGAGGAAGTTAGAGAAGA
gyrB R	GCATATCCACTGTTATATTGAAG
aap F	ACGAGGAATTACAATCATCA
aap R	GTAGTTGGCGGTATATCTATT
Primers used for truncation of the GB1-His-tagged AapBrpt1.5
F^c	CATGCCATGGGCATGCAGTACAAGCTTGCTCTGAACG
TF_1–160 R^a	CCGCTCGAGTTAAACGCGCTCTTCACCTGGTTTTAAA
TF_1–102 R^a	CCGCTCGAGTTAAACTTCAGTTTTACTATCTACAGGTGCA
TF_1–53 R^a	CCGCTCGAGTTATGTTAATGGGTTCTTAGTTGTTGGC
TF_1–132 R^a	CCGCTCGAGTTAACCAACTTTCGGACCATATTTTGT
TF_1–122 R^a	CCGCTCGAGTTAATCCACTGGTGGGGTAACAACT
TF_1–112 R^a	CCGCTCGAGTTAATCAGGATTTTTAACTCCTGGTTTA
TF_1–90 R^a	CCGCTCGAGTTAAAATTCATCTTTATGACCTTGTGGTAT
TF_1–80 R^a	CCGCTCGAGTTATTCACCACCATAATGAACAATCTCAT
TF_1–70 R^a	CCGCTCGAGTTATGGTTGTTTTGTTATTTTTTCTGTTGG
TF_1–60 R^a	CCGCTCGAGTTAACCTTCGCCAACTTTTTCTCCTGTT
Primers used for site-directed mutagenesis of the GB1-His tagged TF₁ _–102
F^a	ACAACCAGTGGATGAGATTACTGAATATGGTGGTGAACAAATACC
R^a	GGTATTTGTTCACCACCATATTCAGTAATCTCATCCACTGGTTGT

a. Primers were designed according to the genomic sequence of S. epidermidis ATCC 12228 (GenBank NC_004461).

b. Primers were designed according to the genomic sequence of S. epidermidis RP62A (GenBank NC_002976).

c. Primers were designed according to the gene sequence of the 56-residue B1 immunoglobulin binding domain (GB1) of immunoglobulin G-binding protein from Streptococcus dysgalactiae subsp. equisimilis GGS_124 (amino acids 303–357, GenBank YP_002997067).