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. 2011 Jun 7;6(6):e20916. doi: 10.1371/journal.pone.0020916

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Lerman et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A–C) The levels of K10 and K14 differentiation markers were assessed in HaCaT cells stably expressing hsa-miR-99a or the HTR control RNA by RT-PCR (A) and qPCR (B–C) analysis. (D) PHK cells were seeded in high, 1.5 mM Ca⁺⁺ medium. Cells were harvested at the indicated time points and the level of endogenous miR-99a was determined by qPCR.