Fig. 2.

N-terminal mhtt aggregates in COS-7 cells. a Transiently transfected COS-7 cells expressing htt-N233-82Q exhibit perinuclear (row 1) and nuclear (row 2, arrowhead) inclusions stained with the N-terminal antibodies htt 81–90 and htt 115–129 or with the antibody htt 81–90 only (row 3–4, arrow). Scale bars are 20 μm. b Distribution of mhtt aggregates in COS-7 cells expressing htt-N233-82Q. Transfected cells with aggregates stained by the antibodies htt 81–90 and htt 115–129 or htt 81–90 alone were counted per coverslip in independent experiments (n = 3)