Fig. 6.

Cell viability of transfected N2a cells expressing mhtt. a Cell viability of N2a cells expressing htt-N233-82Q and its mutant htt-N233-82Q Δ121–122. Transfection of the empty vector pRC/CMV served as control. Cell viability was assessed 48 h after transfection using trypan blue dye solution. In cells expressing the mutant htt-N233-82Q Δ121–122 cell viability was 16.5 % (n = 3, ** P < 0.01) reduced compared to cells expressing htt-N233-82Q. b Aggregate formation in N2a cells expressing either htt-N233-82Q or its mutant htt-N233-82Q Δ121–122. Aggregates of mhtt are labeled with the antibody htt 81–90