Figure 3.

The over-expression of the β2 subunit of proteasome did not alter the level of Rad51p. We determined the level of Rad51p after treatment with 30 μM MG132. (a) 30 μg of total protein extract was loaded as follows: line1: negative control. line 2: extract by cells treated with MG132. We also determined the effect of the β2 subunit over-expression on Rad51p level. (b) 30 μg of total protein extract was loaded as follows: lane 1: total protein extract by control cells; lane 2: extract by cells overexpressing the proteasome subunit. α tubulin was detected as loading control.