Table 3.

Genes Showing Major Expression Changes after Addition of 125 ng/mL of Novobiocin

No.a	Gene product	Fold change mRNA (protein)
10 min	30 min	60 min
hi0944	Riboflavin biosynthesis protein	2.9	2.9	3.6
hi0873	UDP-N-acetylglucosamine epimerase	4.6	4.7	3.4
hi1722	Methionine aminopeptidase	3.5 (NC)	3.9 (NC)	3.4 (3.2)
hi1051	Leukotoxin secretion ATP-binding protein	3.1	3.9	3.3
hi1623	Mercury resistance regulatory protein	3.2	2.7	3.2
hi0991	DNA/ATP binding protein	2.5	2.7	3.1
hi0872	Undecaprenyl-phosphate galactosephosphotransferase	3.6	4.3	3.0
hi1305	Hydrogenase gene region	2.7	3.2	2.9
hi1573	Pyruvate kinase type II	2.3 (NC)	2.3 (NC)	2.7 (NC)
hi0394	Peptidyl-tRNA hydrolase	2.6	2.4	2.6
hi0533	RNA polymerase sigma-70 factor	2.4	2.1	2.6
hi1247	Excinuclease ABC subunit B	2.2	2.4	2.5
hi1304	N utilization substance protein B	2.3	2.9	2.5
hi1441	Stringent starvation protein A	2.3 (NC)	2.9 (2.2)	2.5 (NC)
hi0567	DNA gyrase, subunit B	2.1	2.9	2.4
hi0603	Uroporphyrinogen III methylase	2.7	2.1	2.4
hi1307	Virulence plasmid protein	2.4	2.5	2.3
hi0769	Cell division ATP-binding protein	3.0	2.5	2.3
hi0970	3-Dehydroquinase	2.3	2.2	2.2
hi1264	DNA gyrase, subunit A	2.5	2.1	2.2
hi0992	DNA polymerase III beta-subunit	2.2 (NC)	2.5 (NC)	2.2 (NC)
hi0969	O-Succinylbenzoate-CoA synthase	2.1	2.4	2.2
hi0623	Methionyl-tRNA formyltransferase	3.0	2.8	2.1
hi0625	TRK system potassium uptake protein	3.1	3.0	2.1
hi0236	ArsC protein	−2.4	−2.3	−2.1
hi1734	Enoyl-reductaseb	−2.6 (−8.8)	−2.6 (−7.2)	−2.1 (−6.2)
hi0422	ATP-dependent RNA helicase	−2.8	−2.6	−2.1
hi0537	Urease accessory protein	−2.3	−4.0	−2.1
hi1661	Dihydrolipoamide succinyltransferase	−4.7 (NC)	−2.6 (NC)	−2.2 (NC)
hi0425	Phosphatidylserine synthase	−2.9	−2.1	−2.2
hi0863	Pyridoxamine phosphate oxidase	−3.0	−3.4	−2.2
hi1434.1	Cold shock-like protein	−3.0	−2.1	−2.2
hi1186	Dipeptide transport system permease protein	−2.5	−2.5	−2.2
hi1676	Molybdenum cofactor biosynthesis protein A	−3.9	−3.5	−2.2
hi1040	Restriction enzyme	−3.0	−3.4	−2.2
hi0503	High affinity ribose transport protein	−3.4	−2.9	−2.3
hi1152	PmbA protein homolog	−3.0	−2.2	−2.3
hi1193	Branched-chain-amino-acid transaminase	−2.3 (NC)	−2.5 (−1.5)	−2.3 (NC)
hi0538	Urease accessory protein	−2.9	−3.2	−2.3
hi1033	Phosphoserine phosphatase	−5.0	−2.8	−2.3
hi1632	Lysine-sensitive aspartokinase III	−3.3	−2.4	−2.3
hi1227	Uracil permease	−2.3	−2.8	−2.4
hi1041	Modification methylase HgiDI	−2.8	−2.4	−2.5
hi1163	D-Lactate dehydrogenase	−3.2	−3.2	−2.5
hi0117	Membrane-bound lytic murein transglycosylase A	−2.5	−2.8	−2.6
hi1022	Biotin synthetase	−4.7	−3.4	−2.6
hi0563	Regulatory protein AsnC	−2.7	−2.5	−2.6
hi0539	Urease, alpha subunit	−3.2 (NC)	−3.8 (NC)	−2.6 (NC)
hi1201	Adenine specific methylase, putative	−2.7	−2.9	−2.7
hi1682	Putative protease	−2.9	−2.3	−2.7
hi1065	MukB suppressor protein	−2.5	−2.5	−2.9
hi1545	C4-Dicarboxylate transport protein	−5.0	−3.9	−2.9
hi1383	Periplasmic phosphate-binding protein	−2.1	−4.0	−3.0
hi0078	Cys-tRNA synthetase	−2.6	−2.5	−3.0
hi1263	Homoserine acetyltransferase	−2.7	−2.6	−3.1
hi0419	Collagenase	−4.3	−3.2	−3.2
hi1106	Xylose operon regulatory protein	−2.8	−2.7	−3.2
hi1507	Hypothetical protein	NC (−4.1)	−3.5 (−3.2)	−3.2 (−2.9)
hi1068	Formate-dependent nitrite reductase	−3.0	−2.7	−3.3
hi1735	Peptide-chain-release factor 3	−4.3	−3.3	−3.4
hi1112	Xylose isomerase	−2.3	−3.2	−3.4
hi1019	Thiamin-binding periplasmic protein	−5.0	−4.7	−3.4
hi0445	Protein translocation protein, low temperature	−3.6	−4.6	−3.8
hi0548	Initiation factor IF-1	−7.7	−5.8	−4.0
hi1181	Phosphoheptose isomerase	−4.2	−5.2	−4.0
hi1172	S-Adenosylmethionine synthetase 2c	−3.9 (NC)	−4.1 (3.0)	−4.0 (NC)
hi1166	Histidinol-phosphate aminotransferase	−6.5	−4.1	−4.1
hi1171	Anthranilate synthase component II	−3.8	−3.4	−4.1
hi0501	High affinity ribose transport protein	−2.7	−2.4	−4.1
hi0182	Glucose kinase	−3.1	−5.2	−4.3
hi1728	Branched chain amino acid transport  system II carrier protein	−9.5	−7.5	−4.5
hi1170	Anthranilate synthase component I	−5.7	−4.2	−4.8
hi0549	16S rRNA-dimethyltransferase	−7.6	−5.0	−4.9
hi0808	Ribosome releasing factor	−4.5 (−4.7)	−5.3 (−4.4)	−4.9 (−4.7)
hi0214	Oligopeptidase A	−4.8	−6.8	−5.0
hi1009	Glycerol-3-phosphate regulon repressor	−7.4	−9.8	−5.0
hi0179	Pyruvate formate-lyase activating enzyme	−6.9	−6.1	−5.1
hi0211	Phosphatidylglycerophosphatase B	−4.5	−7.6	−5.7
hi1365	Topoisomerase I	−4.4	−5.8	−6.1
hi1167	Phosphoserine aminotransferase	−9.1	−9.6	−6.2
hi1154	Proton glutamate symport protein	−4.2	−4.1	−6.3
hi1729	Lactam utilization protein	−6.9	−9.3	−6.5
hi1048	Hypothetical protein	−9.1	−14.1	−9.6
hi1035	Magnesium and cobalt transport protein	−9.2	−10.5	−12.2

^aNumbering according to Fleischmann et al. (1995). 

^bPresent as two spots; other spot decreased to a lesser extent. 

^cPresent as two spots; other spot increased in intensity at the 30-min time point.