Table 6.

Genes Showing Major Changes in Expression after Addition of 300 ng/mL of Ciprofloxacin

No.	Gene product	Fold change
10 min	60 min
hi0567	DNA gyrase, subunit B	2.1	1.9
hi1264	DNA gyrase, subunit A	1.8	1.8
hi1365	Topoisomerase I	NC	−3.1
hi0070	DNA repair protein RecN	4.8	13.0
hi0749	LexA repressor	3.9	7.0
hi1349	Neutrophil activating protein	NC (1.7)	6.3 (3.4)
hi0750	Diaminopimelate epimerase	2.2	5.4
hi0965	Ribosomal protein S20	1.7	5.3
hi1188	DNA helicase II	1.9	4.5
hi0249	Excinuclease ABC subunit A	2.3	4.4
hi0715	ATP-dependent protease ATPase subunit (Clpx)	NC (NC)	4.2 (NC)
hi0313	Holliday junction DNA helicase	1.5	3.9
hi0459	Pyrimidine operon regulatory protein	NC (NC)	3.6 (NC)
hi1392	HindIII modification methyltransferase	NC	3.5
hi0332	DNA repair protein (RecO)	NC	3.5
hi0623	Methionyl-tRNA formyltransferase	2.4	3.4
hi0330	Cell envelope protein	1.9	3.3
hi0312	Holliday junction DNA helicase	1.8	3.3
hi0713	Trigger factor	2.1 (NC)	3.3 (1.9)
hi0394	Peptidyl-tRNA hydrolase	NC	3.3
hi1125	Transaldolase B	2.1 (NC)	3.2 (2.1)a
hi0250	Single-stranded DNA binding protein	2.5 (2.0)	2.9 (1.5)
hi0113	Heme-hemopexin utilization protein C	−1.6	2.8
hi0256	Lipoprotein-34	NC	2.8
hi0079	Peptidyl-prolyl cis-trans isomerase B	NC (NC)	2.8 (NC)
hi0872	Undecaprenyl-phosphate galactosephosphotransferase	NC	2.8
hi0970	3-Dehydroquinase	NC	2.8
hi1384	Ferritin like protein	NC	2.7
hi0413	Ribonuclease E	2.4	2.7
hi1742	RNA polymerase omega subunit	1.5	2.7
hi0602	HemY protein homolog	1.6	2.6
hi0717	Transcription antitermination protein NusG	NC	2.6
hi1447	GTP cyclohydrolase I	NC (1.7)	2.6 (NC)
hi0411	Host factor-I	2.1	2.6
hi0287	Tryptophan-specific permease	NC	2.6
hi0331	Opacity associated protein	NC	2.5
hi0991	DNA/ATP binding protein	NC	2.5
hi0622	Polypeptide deformylase	NC	2.5
hi0206	5-Nucleotidase, putative	NC (NC)	2.5 (NC)
hi0603	Uroporphyrinogen III methylase	NC	2.4
hi1325	3-Hydroxydecanoyl-(acyl carrier-protein) dehydratase	NC	2.4
hi0993	Chromosomal replication initiator protein	NC	2.4
hi0865	Glutamine synthetase	1.6	2.4
hi1561	Polypeptide chain release factor 1	NC (NC)	2.3 (NC)
hi1304	N utilization substance protein B	1.8	2.3
hi1740	DNA recombinase RecG	NC	2.3
hi1305	Hydrogenase gene region	NC	2.2
hi0881	Octaprenyl-diphosphate synthase	NC (NC)	2.2 (NC)
hi1385	Ferritin like protein	NC (1.4)	2.2 (NC)
hi0927	Glycyl-tRNA synthetase alpha chain	NC (NC)	2.1 (1.7)
hi0921	Leucyl-tRNA synthetase	2.1	2.1
hi0718	VacJ lipoprotein	NC	2.1
hi0689	Glycerophosphoryl diester phosphodiesterase	NC	2.1
hi0768	Cell division protein FtsY	NC (NC)	2.1 (NC)
hi0624	Fmu/Fmv gene product	2.1	2.1
hi0600	DNA recombinase RecA	2.7	2.0
hi0567	DNA gyrase, subunit B	2.1	1.9
hi1220	Ribosomal protein Sl	3.3	1.9
hi0785	Ribosomal protein L29	2.6	NC
hi0516	Ribosomal protein L1	2.0	NC
hi0782	Ribosomal protein L22	3.0	NC
hi0779	Ribosomal protein L23	2.2	NC
hi0571	Hydrogen peroxide-inducible activator	NC	−2.1
hi0150	Lambda cII stability-governing protein	NC	−2.1
hi0629	Negative rpo regulator	NC	−2.1
hi1135	Phospho-N-acetylmuramoyl-pentapeptide-transferase E	−2.2	−2.1
hi1251	Virulence associated protein A	NC	−2.2
hi0508	Menaquinone biosynthesis protein	NC	−2.2
hi0538	Urease accessory protein	−1.6	−2.2
hi0506	rbs repressor	NC	−2.2
hi1661	Dihydrolipoamide succinyltransferase	NC (NC)	−2.2 (1.7)
hi0546	Primosomal protein N presursor	NC	−2.2
hi0239	Protein-export membrane protein SecF	−2.0	−2.3
hi0745	L-Asparaginase II	NC (NC)	−2.3 (−2.0)
hi0470	Histidinol-phosphate aminotransferase	NC	−2.3
hi0008	Formate dehydrogenase-O gamma subunit	NC	−2.3
hi1133	UDP N-acetylmuramyl-tripeptide synthetase	NC	−2.3
hi0140	N-Acetylglucosamine-6-phosphate deacetylase	NC	−2.4
hi0117	Membrane-bound lytic murein transglycosylase A	−1.8	−2.4
hi1154	Proton glutamate symport protein	−1.9	−2.4
hi1218	L-Lactate permease	NC	−2.4
hi0537	Urease accessory protein	NC	−2.4
hi0373	Heat shock cognate 66	NC	−2.5
hi0142	N-Acetylneuraminate lyase	NC	−2.5
hi1227	Uracil permease	−2.3	−2.5
hi0505	Ribokinase	NC	−2.6
hi0544	Ribosomal protein L9	NC (NC)	−2.6 (NC)
hi1167	Phosphoserine aminotransferase	NC	−2.6
hi0536	Urease accessory protein	NC	−2.6
hi0087	Threonine synthase	NC (−2.6)a	−2.7 (NC)
hi0089	Aspartokinase I/homoserine dehydrogenase I	NC (NC)	−2.8 (NC)
hi1367	Threonyl-tRNA synthetase	NC (NC)	−2.8 (NC)
hi1511	Sheath protein Gpl	NC	−2.8
hi0447	L-Phosphofructokinase	NC	−2.8
hi1172	S-Adenosylmethionine synthetase 2	NC (−1.3)	−2.8 (−1.9)b
hi0564	Asparatate ammonia ligase	NC (NC)	−2.8 (NC)
hi0088	Homoserine kinase	NC (NC)	−2.9 (NC)
hi1545	C4-dicarboxylate transport protein	−2.4	−2.9
hi0189	Glutamate dehydrogenase	NC	−3.0
hi0465	Phosphoglycerate dehydrogenase	NC (NC)	−3.1 (NC)
hi0446	Fructose-permease IIBC component	−2.3	−3.1
hi1727	Argininosuccinate synthetase	NC (NC)	−3.2 (NC)
hi1181	Phosphoheptose isomerase	NC	−3.3
hi1177	Arginine transport system permease protein	−2.9	−3.4
hi0548	Initiation factor IF-1	−2.7	−3.4
hi0141	Glucosamine-6-phosphate isomerase	NC	−3.7
hi0913	Ribosomal protein S2	NC (NC)	−3.9 (−4.3)
hi1179	Periplasmic arginine-binding protein	NC (NC)	−4.0 (NC)
hi0509	Menaquinone biosynthesis protein	NC	−4.0
hi0628	RNA polymerase sigma-E factor	NC	−4.0
hi1636	Phosphoenolpyruvate carboxylase	NC	−4.4
hi1178	Arginine transport system permease protein	−2.1	−4.6
hi1729	Lactam utilization protein	NC	−5.2
hi1728	Branched chain amino acid transport system II carrier protein	−2.6	−5.5
hi1180	Arginine transport ATP-binding protein	−2.2	−6.4

^aOnly one of two spots changed in intensity. 

^bThe second spot corresponding to this protein was increased.