Figure 2.

A, Agarose electrophoresis showing detection of Paragonimus kellicotti DNA in clinical specimens by polymerase chain reaction. Lane 1, no template control; lane 2, template was genomic DNA from a non-infected human; lane 3, template DNA isolated from a lung biopsy specimen from a patient with paragonimiasis; lane 4, 100-basepair DNA marker; lane 5, template DNA isolated from the sputum of a patient with paragonimasis that contained P. kellicotti eggs; DNA was isolated from metacercariae isolated from Missouri crayfish. B, Western blot using P. kellicotti native antigen probed with serum samples obtained from experimentally infected gerbils. Lanes 1–3, serum samples from three gerbils that died 41, 39, and 45 days after intraperitoneal injection with four or five metacercariae, respectively. All gerbils had adult worms in lungs and eggs in the pleural cavity at necropsy; lane 4, serum sample from 25 days post-infection from the same gerbil that was tested in lane 3; lanes 5–7, serum samples from uninfected control gerbils.