FIG 5 .

Mutations blocking glucose utilization produce varied effects on ATP production in ex vivo human CSF. WT and mutant strains of C. neoformans were grown in YPL shake culture at 37°C to saturation, then centrifuged, washed with sterile PBS, and resuspended in filter-sterilized pooled human CSF. These CSF shake cultures were then incubated at 37°C, with 15-ml aliquots removed at 1 h and 24 h, and processed as described elsewhere (67). (A) Measurement of ATP levels in the WT, pyk1∆, pyk1∆ PYK1, pyk1∆/mig1∆, and mig1∆ strains following exposure to ex vivo CSF for 1 h or 24 h. Differences between mutant strains and the WT at each time point were compared using Student’s t test (*, P < 0.005). (B) Measurement of ATP levels in the WT, hxk1∆, hxk2∆, and hxk1∆/hxk2∆ strains following exposure to ex vivo CSF for 1 h or 24 h. Differences between mutant strains and the WT at each time point were compared using Student’s t test (α = 0.05).