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IRMPD tandem MS was applied to elucidate O-linked oligosaccharide structures. (A) The mass spectrum of one HPLC fraction (F12) from X. borealis egg jelly, (B) glycan ion (m/z 1024, [M + Na]+) peak isolated in the ICR cell, and (C) IRMPD mass spectrum yielding fragments from the precursor ion (m/z 1024), enabling structural elucidation of the oligosaccharide.
