. 2011 Mar 18;21(7):934–948. doi: 10.1093/glycob/cwr026

Table II.

Positive-ion MALDI-TOF analysis of oligosaccharides derived from the SCWPs from B. anthracis and pathogenic B. cereus

Strains	B. anthracis^a			B. cereus G9241			B. cereus 03BB102
Strains	Gal #	^b[M + Na]⁺	%^c	Gal #	^b[M + Na]⁺	%^c	Gal#	^b[M + Na]⁺	%^c
One repeat^d							1	812.3	68.9
	2	974.3	63.0				2	974.4	60.0
	3	1136.4	100.0	3	1136.4	100.0	3	1136.5	100.0
				4	1298.6	185.0	4	1298.5	155.6
							5	1460.7	97.8
Two repeats^d							2	1583.7	86.7
	3	1745.6	3.3	3	1745.9	40.3
	4	1907.6	13.2	4	1907.9	112.9
	5	2069.7	22.0	5	2069.9	145.2	5	2069.8	24.4
	6	2231.7	16.5	6	2231.9	153.2	6	2231.9	26.7
				7	2394.0	129.0	7	2393.9	33.3
				8	2556.1	64.5	8	2556.0	35.6
							9	2718.0	24.4
							10	2880.1	9.3
Three repeats^d	6	2842.8	1.6	6	2842.2	16.1
	7	3002.8	5.5	7	3003.2	33.1
	8	3164.8	4.4	8	3167.2	32.2
	9	3327.8	1.5	9	3327.3	24.2
				10	3492.2	17.7
				11	3652.4	12.1
Four repeats^d							4	3126.2	11.6
							5	3288.2	13.6
							7	3613.4	5.6

The variation in galactose substitution of single and multiple repeating oligosaccharide units of the SCWP from strains of B. anthracis, and B. cereus strains G9241 and 03BB102 are compared.

^aThe SCWP-derived oligosaccharides from B. anthracis Ames, Sterne and Pasteur all give identical results.

^bThe observed ion mass.

^cIon intensity expressed relative to ion m/z 1136.4 (assigned as 100%). The spectrum of the Bc03BB87-derived oligosaccharides was virtually identical to that of BcG9241.

^dFractions containing single, double and higher repeating units of the respective SCWPs were obtained by size exclusion chromatography on Bio-Gel P-2. Each repeating unit consists of three HexNAc residues and a variable number of Gal residues, e.g. the observed sodiated mass for one repeat with Gal #2 = 974.3; predicted mass = 973.87, calculated from the average incremental masses as described in Materials and methods.