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. Author manuscript; available in PMC: 2012 Jun 15.
Published in final edited form as: J Immunol. 2011 May 20;186(12):7089–7097. doi: 10.4049/jimmunol.1100565

Figure 5. S. aureus and Pam3Cys induced cytokine secretion in Muller glia.

Figure 5

MIO-M1 cells were stimulated with Pam3Cys (10 μg/ml), live S. aureus (SA) or heat-killed S. aureus (HKSA) for indicated time points. Culture supernatants were used to quantitate indicated cytokines/chemokines accumulation by ELISA and cells were lysed to determine protein concentration. The levels of cytokines are expressed in picogram/milligram (pg/mg) of cell lysate and the results represent data (mean ± SD) from three independent experiments performed in triplicate. Statistical analysis was performed using Student’s t-test and indicated statistical differences are comparisons of stimulated vs unstimulated control (C): *p < 0.05; **p < 0.001.