Figure 3.
High power (100X objective) illustrations of the variety of varicose and lamellar appositions formed by IMAs on ICCs. The complexity of apparent appositions between IMAs and ICCs ranges from elaborate and lamellar varicosities (Panels A, B and C) to subtle dilations or swellings of relatively smooth neurites coursing along the ICCs (Panels D and E). Panel A: a labeled IMA is shown without ICC counterstaining in order to accentuate the complexity of lamellar varicosities observed. Panels B, C, D and E illustrate the range of complexity of tracer-labeled IMA appositions (DAB processing, brown) seen in conjunction with ICCs (cKit immunohistochemistry with Vector VIP, violet). Scale bar in Panel E, for all panels: 10 μm.