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. Author manuscript; available in PMC: 2012 Jun 15.
Published in final edited form as: J Immunol. 2011 May 13;186(12):7156–7163. doi: 10.4049/jimmunol.1002097

Figure 4. Impaired vascular activation and local TNF production in LIGHT−/− mice after CFA immunization.

Figure 4

A. WT or LIGHT−/− mice were immunized with CFA, 22–24 hrs later, skin on top of the CFA site was collected and RNA was isolated. SLC and ELC expression level was determined by quantitative PCR. *, P < 0.05. Representative of two experiments. B. WT or LIGHT−/−mice were immunized with CFA or left untreated, 22–24 hrs later, LNs from each group were pooled and collagenase digested; CD45-CD31+ cells were sorted by flow cytometry and RNA was isolated. VCAM-1 expression level was determined by quantitative PCR. **, P < 0.01. Data were representative from two experiments. C. Skin homogenate was made 22hrs after CFA immunization from either WT or LIGHT−/− mice, TNF production was measured by CBA. *, P < 0.05. Representative of two experiments. D. TNFR-hIgG or control IgG was injected i.d. locally at the tail base, followed immediately by CFA immunization. iLN cellularity was determined at day 3. *, P < 0.05. Representative of two experiments.