Figure - PMC

Skip to main content

View full-text article in PMC

. Author manuscript; available in PMC: 2012 Aug 1.

Published in final edited form as: Biochim Biophys Acta. 2011 Apr 22;1808(8):2045–2050. doi: 10.1016/j.bbamem.2011.04.007

Fig 1 — Immmunostaining of HEK 293T cells expressing wild-type and mutant FGFR3 at the plasma membrane. Cells were cultured for 24 hours after transfection and serum starved. After fixing with 4% paraformaldehyde (PFA), the cells were blocked using bovine serum albumin (BSA) for 1 hour. The cells were incubated with anti-FGFR3 antibodies, followed by FITC-conjugated goat anti rabbit IgG antibodies without cell permeabilization to identify wild-type and mutant receptors localized at the cell surface. (A) 10× objective; (B) 60× objective.

Fig 1 — Immmunostaining of HEK 293T cells expressing wild-type and mutant FGFR3 at the plasma membrane. Cells were cultured for 24 hours after transfection and serum starved. After fixing with 4% paraformaldehyde (PFA), the cells were blocked using bovine serum albumin (BSA) for 1 hour. The cells were incubated with anti-FGFR3 antibodies, followed by FITC-conjugated goat anti rabbit IgG antibodies without cell permeabilization to identify wild-type and mutant receptors localized at the cell surface. (A) 10× objective; (B) 60× objective.