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. 2011 Jun 8;6(6):e20216. doi: 10.1371/journal.pone.0020216

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Brim et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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The presence of visible PCR product in lanes “U” indicates the presence of unmethylated genes of SLC5A8, the presence of product in lanes “M” indicates the presence of methylated genes. Water controls for PCR reaction are also shown as NT: no template. MSP of SLC5A8 in NB: Normal Blood lymphocyte DNA used as control for unmethylated at SLC5A8, SW48: colon cancer cell line used as a positive control. Patients # 3–5 are methylated, and patients # 1–2 are semimethylated.