Figure 2.

(Top line) Binary sequence pattern of a designed combinatorial library of de novo proteins. The sequences are 63 residues long and are designed to form six β-strands (arrows) punctuated by turns. (Second line) Alternating pattern in the β-strands is indicated with polar residues (○) as white font on black background and nonpolar residues (●) as black font on gray background. Combinatorial diversity is incorporated at positions marked ○, ●, and t (turn). Fixed residues are incorporated at the termini and in some of the turns (18, 19). (Lines 3–5) Amino acid sequences (single-letter code) of three de novo proteins from the combinatorial library.