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. Author manuscript; available in PMC: 2012 Nov 1.
Published in final edited form as: Cardiovasc Pathol. 2011 Feb 8;20(6):325–333. doi: 10.1016/j.carpath.2010.10.001

Figure 2.

Figure 2

Message and protein expression and cellular morphology in released versus constrained gels cultured in M199 supplemented with 5% FBS for 6-48 h. Panel A shows gene expression of col1a1, col3a1, TGF-β, and α-SMA in released (left panels) or constrained (right panels) fibroblast-seeded gels. Panel B shows merged images of constrained (left panel) and released (right panel) collagen gels stained for DAPI (blue), actin (red), and α-SMA (green). Panel C shows quantification of the immunofluorescence images shown in Panel B, using a circularity parameter.