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. Author manuscript; available in PMC: 2012 Jun 15.
Published in final edited form as: J Immunol. 2011 May 4;186(12):6839–6847. doi: 10.4049/jimmunol.1002721

FIGURE 1.

FIGURE 1

The cytoplasmic tail of CD3 ζ contains multiple clusters of arginine and lysine residues that complex phosphoinositides. A, Amino acid sequence of the cytoplasmic tail of murine CD3 ζ containing clusters of basic amino acids, as indicated by asterisks. Individual biotinylated peptides containing the three BRS sequences (BRS-1, BRS-2, BRS-3) and the three ITAMs (ITAM-1, ITAM-2, ITAM-3) are shown. The tyrosine residues present in the ITAMs are underlined. B, PIP-strips embedded with diverse lipids were probed with the biotinylated peptides. Binding was detected by streptavidin-HRP western blotting, with the different exposure times indicated below the membrane. Data are representative of 4 independent experiments. C, Biotinylated peptides containing ITAM-1, BRS-2, and BRS-3 were used to immunoblot lipid arrays containing serial dilutions of the indicated phospholipids ranging from 100 to 1.56 pmoles, as shown in the left panel. Peptide binding was assessed as in B. The data are representation of at least 4 independent binding assays per peptide.