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. 2011 Jun 8;6(6):e20309. doi: 10.1371/journal.pone.0020309

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This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration, which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

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(A) A highly conserved putative TEAD-binding site (yellow boxes) is present in the 5′ regulatory region of the pax3 gene in 15 different vertebrates. A previously described mouse TEAD-binding site (red box) appears less conserved. (B) Chromatin isolated from 300 wild type stage 14–16 Xenopus laevis embryos was sheared to a size range of 150 to 900 base pairs. (C) Chromatin immunoprecipitations (ChIPs) from 12.5 µg or 25 µg of sheared chromatin immunoprecipitated a band at the expected size for the putative novel TEAD1-binding site region with the hYAP antibody but not with a control IgG antibody. (D) Sequencing of this band from three different clones verified that the genomic region pulled down by the hYAP anitbody contained the novel TEAD-binding site (yellow) when compared to the Xenopus tropicalis genomic sequence.