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. Author manuscript; available in PMC: 2011 Dec 1.
Published in final edited form as: Nat Med. 2011 May 29;17(6):726–731. doi: 10.1038/nm.2380

Figure 2. Early proliferation defect and the absence of a postnatal Shh defect.

Figure 2

(a) BrdU stained (green) sections from representative littermates aged E16.5 and E18.5. Dashed lines demarcate external granule layer and Hoechst labels nuclei. Scale bar, 50 μm. (b) Quantification of BrdU labeled CGNs indicated as average number of BrdU positive cells as a ratio of total CGNs. E16.5, and to a lesser extent P5, reveal a significant decrease in relative BrdU stained cells. *P<0.05, **P<0.005 Student’s t-test, n = 3 for each genotype. (c) N-myc staining (green) in midline sagittal sections from P5 littermates. Hoechst labels nuclei. Scale bar, 20 μm (d) qRT-PCR analysis of Shh target genes, N-Myc, Gli1, and Ptc1. Values are average expression levels relative to actin. NS=not significant, Student’s t-test, n = 3 for each genotype and gene tested. Error bars are S.E.M.