Table 1.
Description of antibodies used in this study.
| Antibody | Company | Dilution Used | Technique |
|---|---|---|---|
| β-actin (AC-74) | Sigma | 1:10,000 | WB |
| Akt (B-1) | Santa Cruz Biotechnology | 1:200 | WB |
| BAX (Δ 21) | Santa Cruz Biotechnology | 1:200 | WB |
| Bcl-2 (C-21) | Santa Cruz Biotechnology | 1:100 | IF |
| Calcineurin (H209) | Santa Cruz Biotechnology | 1:100 | IF |
| CDK4 (H-22) | Santa Cruz Biotechnology | 1:200 | WB |
| CDK6 (C-21) | Santa Cruz Biotechnology | 1:200 | WB |
| Cyclin D1 (HD11) | Santa Cruz Biotechnology | 1:200 | WB |
| Cyclin D3 (D7) | Santa Cruz Biotechnology | 1:200 | WB |
| Cytokeratin 1 | Santa Cruz Biotechnology | 1:100 | IF |
| Cytokeratin 10 | Abcam | 1:500 | IF |
| E-cadherin (H108) | Santa Cruz Biotechnology | 1:100, 1:500 | IF, WB |
| Fibronectin (C-20) | Santa Cruz Biotechnology | 1:100 | IF |
| Goat anti mouse Dylight 488 | Pierce | 1:500 | IF |
| Goat anti-mouse AlexaFluor 594 | Invitrogen | 1:500 | IF |
| Goat anti-mouse HRP | Pierce | 1:8,000 | WB |
| Goat antirabbit AlexaFluor 594 | Invitrogen | 1:500 | IF |
| Goat anti-rabbit Dylight 488 | Pierce | 1:500 | IF |
| Goat anti-rabbit HRP | Pierce | 1:8,000 | WB |
| VEGF (C-1) | Santa Cruz Biotechnology | 1:200, 1:100 | WB, IHC |
| Vimentin (C-20) | Santa Cruz Biotechnology | 1:100 | IF |
| MMP-2 (8B4) | Santa Cruz Biotechnology | 1:100, 1:500 | IF, WB |
| MMP-9 (C-20) | Santa Cruz Biotechnology | 1:100, 1:500 | IF, WB |
| Mouse anti-goat Fluorescein | ThermoScientific | 1:500 | IF |
| Mouse anti-goat HRP | Pierce | 1:8,000 | WB |
| N-cadherin (H63) | Santa Cruz Biotechnology | 1:100, 1:500 | IF, WB |
| NFAT (7A6) | Santa Cruz Biotechnology | 1:100 | IF |
| α-SMA (1A4) | Sigma | 1:100, 1:500 | IF, WB |
| Fibronectin | Abcam | 1:1000 | WB |
| PCNA (C-20) | Santa Cruz Biotechnology | 1:100 | IHC |
| PCNA (PC10) | Abcam Incorporated | 1:5000 | WB |
| p-Smad 2/3 (Ser423/425) | Santa Cruz Biotechnology | 1:100 | IF |
| Rabbit anti-goat AlexaFluor 594 | Invitrogen | 1:500 | IF |
| Smad 2 (Y2-13) | Santa Cruz Biotechnology | 1:100, 1:500 | IF, WB |
| Smad 4 (B-8) | Santa Cruz Biotechnology | 1:100, 1:500 | IF, WB |
| Smad 7 (Z8B) | Santa Cruz Biotechnology | 1:100, 1:400 | IF, WB |
| Snai | Abcam | 1:1000 | WB |
| TGFβRI (T-19) | Santa Cruz Biotechnology | 1:100, 1:500 | IF, WB |
| TGFβRII (C-21) | Santa Cruz Biotechnology | 1:100, 1:500 | IF, WB |
| Twist (Twist 2C1A) | Santa Cruz Biotechnology | 1:100 | IF |