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Two different proposed T4HN biosynthetic pathways catalyzed by PKS1. a Proposed biosynthetic pathway using mal-CoA as a starter unit and metabolites isolated from hetrologous expression of PKS1 in A. oryzae M-2-3. b Revisit the product distribution produced by wild type and TE/CLC less PKS1 by LC-ESI-MS. c Alternative proposed mechanism of T4HN (11) formation using acetyl-CoA as a starter unit. *: 14C-labeled carbon form acetyl-CoA
