Table 1.
PCR Primer and TaqMan Probe Sequences for SNP Markers Studied
| Marker | NCBI assay ID | Allele 1 | Allele 2 | Allele 1 (VIC) TaqMan probe | Allele 2 (FAM) TaqMan probe | PCR Primer 1 | PCR Primer 2 |
|---|---|---|---|---|---|---|---|
| D4S2341 | 231 | C | G | VIC-ccatgGgttagccaa-NFQ-MGB | FAM-ccatgCgttagcc-NFQ-MGB | GAGGTTTCACTGGCTTGTGCT | CATGAGACATTTATCTAATGATTTTTTCTTATT |
| D10S1188 | 241 | A | C | VIC-tgtaccacTctttttc-NFQ-MGB | FAM-taccacGctttttc-NFQ-MGB | TGCTGAATTTCCATCTCTGAGTTC | GCAGGATTCAGTGCCAGAAAG |
| D2S1301 | 221 | A | G | VIC-ttcatctccAtttgtg-NFQ-MGB | FAM-tttcatctccGtttgtg-NFQ-MGB | CCCCAGTATCTGGCACATC | TTTCTCAGGTTTCAGGGATTAGA |
| D11S1964 | 245 | del | T | VIC-caccccaatagca-NFQ-MGB | FAM-caccccTaatagca-NFQ-MGB | TAAAGATGTTGGAGAACTGAAAAAGA | GACCACGGAAGGACACATACAT |
| D7S8 | 870137 | C | T | VIC-ctttccCggaatgc-NFQ-MGB | FAM-cctttccTggaatgc-NFQ-MGB | CTCAGGGACCCTGACCTTATTG | GCCTTCTCAGAGCTTATATTATTGTGTC |
| D10S1257 | 243 | T | C | VIC-tgctgttcTtctgcc-NFQ-MGB | FAM-tgttcCtctgcctcc-NFQ-MGB | CCCCAGGCTGCACAGAAC | TCCTTGAGCCTAAGAGATAGGGC |
| WIAF1177 | 686 | T | C | VIC-cccAaagaaaaca-NFQ-MGB | FAM-cccGaagaaaacaa-NFQ-MGB | TGGGGCTTGACTTTCCAA | CAAATGCCTAATAGACACTATTGGTAGAT |
| WIAF1100 | 667 | G | A | VIC-cctacttacGatcctgg-NFQ-MGB | FAM-cctacttacAatcctgg-NFQ-MGB | TGTGGGACCTGAGCACTTTT | GACCACGCTAAACCCAAATACA |
| WIAF1244 | 693 | T | C | VIC-ccccAttgacatcg-NFQ-MGB | FAM-ccccGttgacatc-NFQ-MGB | TCCAAACTTCACTCCATCTCAA | GGGCCAGCCTCTTTTCAT |
| WIAF1296 | 701 | G | A | VIC-actgcctGgcccaa-NFQ-MGB | FAM-actgcctAgcccaaa-NFQ-MGB | AAAAGAAGAAAAAATTTAAAGGGTGTACTTATA | AATGAGGGCTGATCCTACCAC |
| WIAF1430 | 674 | G | A | VIC-ctccagagGtcaagtc-NFQ-MGB | FAM-ctccagagAtcaagtc-NFQ-MGB | GGTCCTCTTTGCTCCTAACATCTAT | TCAAGTAATCACTCACCAGTGTTGA |
| WIAF1504 | 3188 | A | G | VIC-tggctTatactgccc-NFQ-MGB | FAM-tggctCatactgcc-NFQ-MGB | CCGGCTGATTTTCTTCGACA | CCTAGGCCTCATCTGTGGGAA |
| WIAF1842 | 3388 | A | G | VIC-ccattTgttacatttc-NFQ-MGB | FAM-cattCgttacatttca-NFQ-MGB | CATTCTGGTTTTGACATCAGCATT | GCAAAAGGTGCCATGGTGTT |
| WIAF1634 | 2043 | C | T | VIC-aagattGacaattcc-NFQ-MGB | FAM-agaagattAacaattcc-NFQ-MGB | AGGTATTTGAACTTCATTATCTTCAGC | ACATCAATTGAGCTAATGCAAGAA |
| WIAF1761 | 3308 | G | A | VIC-agcCctgttccctt-NFQ-MGB | FAM-cagcTctgttccctt-NFQ-MGB | GATGGTGGAAGCAGCATGAAA | CTGAGGGTGGCGTCTTTAGAGA |
| WIAF2081 | 1194 | T | C | VIC-cacatcaTgtatgcaatg-NFQ-MGB | FAM-cacatcaCgtatgcaa-NFQ-MGB | GCAGATCAGTCAGCCCACTT | CCCCATCCTACCAGTTGGT |
| WIAF2117 | 1230 | C | T | VIC-cctcaCagtctctct-NFQ-MGB | FAM-cctcaTagtctctctc-NFQ-MGB | CCCTTGGCTCCTCCACTT | ACCACCAGTAGCCATCTTGTTT |
| WIAF2188 | 3596 | G | A | VIC-aacacaGaaacccagcc-NFQ-MGB | FAM-aacacaAaaacccagcc-NFQ-MGB | CATGCCCGAATTTAGATGCT | AGAGCTCAGCGTGCTAGTCAT |
| WIAF270 | 2619 | G | A | VIC-atgccCcaagcag-NFQ-MGB | FAM-tgccTcaagcagc-NFQ-MGB | GGCAAAGAACAGAAGTAAAATCCAGAA | CAGTTTCACAGTGAAAGTTGGCAA |
| WIAF2072 | 1185 | G | A | VIC-caccattagGtcctg-NFQ-MGB | FAM-caccattagAtcctg-NFQ-MGB | GAAACGCATCTCACTGTCATTCTATT | CACCATACTTCATGGCAAGGACT |
Some of TaqMan probes were designed for the antisense sequence (NCBI Assay ID ⧣231, 241, 686, 693, 3188, 3388, 2043, 3308, and 2619). The bases under the Allele 1 and Allele 2 columns were those reported in the dbSNP entries.