Table 2.
5′-Nuclease Assay Results for Marker D2S1301 Detected by FP Changes
| Sample | Normalized FAM | Normalized VIC | VIC/FAM | GENOTYPE |
|---|---|---|---|---|
| PD0001 | 11.2 | 8.8 | 0.8 | 1,2 |
| PD0002 | 5.2 | 80.3 | 15.4 | 2 |
| PD0003 | 8.8 | 80.2 | 9.1 | 2 |
| PD0004 | 8.2 | 74.8 | 9.1 | 2 |
| PD0005 | 14.8 | 12.1 | 0.8 | 1,2 |
| PD0006 | 18.3 | 19 | 1 | 1,2 |
| PD0007 | 17.8 | 13 | 0.7 | 1,2 |
| PD0008 | 22.7 | 15.1 | 0.7 | 1,2 |
| PD0009 | 11.8 | 74.7 | 6.3 | 2 |
| PD0010 | 8.2 | 73.8 | 9 | 2 |
| PD0011 | 6.8 | 74 | 10.9 | 2 |
| Neg Ctrl | 242.6 | 128.7 | 0.5 | 0 |
| PD0012 | 11.8 | 79 | 6.7 | 2 |
| PD0013 | 20.8 | 14.8 | 0.7 | 1,2 |
| PD0014 | 21.4 | 14.3 | 0.7 | 1,2 |
| PD0015 | 20.5 | 11.6 | 0.6 | 1,2 |
| PD0016 | 3.8 | 74.5 | 19.6 | 2 |
| PD0017 | 7.4 | 74.9 | 10.1 | 2 |
| PD0018 | 6.6 | 75.7 | 11.5 | 2 |
| PD0019 | 11.5 | 74.7 | 6.5 | 2 |
| PD0020 | 31.8 | 19.7 | 0.6 | 1,2 |
| PD0021 | 6.8 | 74.3 | 10.9 | 2 |
| PD0022 | 82.7 | 6.3 | 0.1 | 1 |
| PD0023 | 19.2 | 14.4 | 0.8 | 1,2 |
| PD0024 | 22.4 | 12.6 | 0.6 | 1,2 |
| Neg Ctrl | 245 | 128.5 | 0.5 | 0 |
| PD0025 | 23.8 | 13.7 | 0.6 | 1,2 |
| PD0026 | 23 | 13.2 | 0.6 | 1,2 |
| PD0027 | 23.3 | 13.3 | 0.6 | 1,2 |
| PD0028 | 91.4 | 5.8 | 0.1 | 1 |
The results of the first 30 samples of the experiment are shown.