. Author manuscript; available in PMC: 2011 Jun 8.

Published in final edited form as: Science. 2010 Nov 5;330(6005):831–835. doi: 10.1126/science.1191175

Table 1.

In vitro and bacterial cell–based assays for β-glucuronidase activity and inhibitor efficacy. k_cat, catalytic rate; K_m, Michaelis constant; N/A, not applicable. N.E., not effective. Errors represent standard deviation, where N = 3.

	E. coli β-glucuronidase in vitro			E. coli cell–based
	IC₅₀ (nM)	k_cat/K_m (s⁻¹ μM⁻¹)	K_i (nM)	EC₅₀ (nM)
No inhibitor	N/A	0.134 ± 0.0123	N/A	N/A
Glucaro-δ-lactam	26400 ± 483	N/A	7750 ± 475	N.E.
Inhibitor 1	283 ± 26.1	0.0987 ± 0.00621	164 ± 13.0	17.7 ± 7.42
Inhibitor 2	369 ± 2.51	0.119 ± 0.00473	208 ± 25.4	28.3 ± 2.11
Inhibitor 3	586 ± 31.1	0.136 ± 0.00633	684 ± 81.6	233.2 ± 2.99
Inhibitor 4	1060 ± 3.54	0.122 ± 0.0205	1380 ± 166	1322.8 ± 1.15