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Validation of the expression of candidate biomarker proteins by Western immunoblotting. Tissue lysates were prepared from matched pairs of fresh-frozen normal (N) and squamous lung tumor (T) tissues. Three representative pairs of tissues were used for each immunoblotting. Amount of protein loaded in each lane was 40 microgram. One of the lanes was added with molecular weight marker in each gel. Immunoblotting of β-actin was performed as a loading control.
