Figure 3. Pedigree for sister derived lines with altered FeGB qualities.

High and low FeGB derivatives were generated from IBM RI Line #039 using backcrossing to both B73 and Mo17 parents. Circles denote maternal parents and squares are paternal parents, where the numbers that appear next to the circles or square refer to the field entry (e.g. 02-095 indicates NY2002 row 095). Caco-2 phenotyping was utilized at four points in this process: for the evaluation of RI lines (Figure 2), to validate the QTL model (Table 7), and to assist derivation of new inbreds (Table 8). The backcrossing program was initiated three years before Caco-2 phenotyping of the RI lines took place. Individuals with altered FeGB qualities are denoted with filled black circles (high FeGB) or gray circles (low FeGB). While not shown here, lines have been advanced to the BC₂S₆ (highly inbred) generation in the NY2010 field season, with an additional round of Caco-2 phenotyping occurring in early 2011.