Table 8. Multi-site evaluation of FeGB in derived lines.

Site-Year	High B73	Low B73	High Mo17	Low Mo17	F-score
IA-2008	0.671 b	0.459 c	0.813 a	0.597 b	15.727
IL-2007	1.573 a	0.974 b	1.411 a	1.055 b	12.370
MO-2008	1.099 b	0.974 b	1.582 a	1.410 a	17.672
MX-2008	1.100 a	0.782 b	n.d.	n.d.	40.916
NC-2008	0.809 a	0.573 c	0.740 ab	0.671 b	7.961
NY-2007	0.923 a	0.791 b	0.870 ab	0.600 c	12.524
NY-2008	1.208 a	0.379 b	1.238 a	0.490 b	37.247
PA-2008	0.495 ab	0.344 b	0.598 a	0.448 ab	3.762

Contrasting BC₂S₄ derivatives from the IBM039 RI line were grown on 8 plots over 2 years, to evaluate the heritability and penetrance of the high FeGB effect across multiple environments. ANOVA were used to assess whether pairs of related high and low-nutritional value derivatives were significantly different and are denoted by letter. Comparisons were made within sites only, where trait data are expressed as a percentage of the control variety from the Caco-2 bioassay. Locations where significant differences were not observed according to our hypotheses appear in italic type.