Table 1. Oligonucleotides used for amplification of UPS and DWN regions of the TcSub2 gene.

Oligonucleotides	Sequence (5′- 3′)	Enzyme	bp
UPS-NEO F	GGGGTCGACGGTGTTATTTGCGTCACGATGTGC	Sal I	700
UPS-NEO R	GGGCGTCGACTTGCTTATGTCTATCTGGTTTCTTA	Sal I
UPS HIGRO F	GGGTCTAGAGGTGTTATTTGCGTCACGATGTGC	Xba I
UPS HIGRO R	GGGCTCTAGATTGCTTATGTCTATCTGGTTTCTTA	Xba I
DOWN-NEO F	GGGGAATTCGATTTAAGGCCTCTGGAAAATAAGAATGAGG	EcoR I	450
DOWN-NEO R	GGGGAATTCAACACAAAAATAAGAAGAAGTATTGGAATGGCAATTGC	EcoR I
DOWN-HIGRO F	GGGGGATCCGATTTAAGGCCTCTGGAAAATAAGAATGAGG	Bam HI
DOWN-HIGRO R	GGGAAGCTTAACACAAAAATAAGAAGAAGTATTGGAATGGCAATTGC	Hind III

NEO: neomycin; HIGRO: hygromycin. bp base pair. The enzyme cleavage sites are in bold.