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. 2011 Jun 8;6(6):e20618. doi: 10.1371/journal.pone.0020618

Figure 5. Temporal and spatial expression of the AtCDC20.1 and AtCDC20.2 genes during plant development.

Figure 5

(A) to (H) Expression patterns of the AtCDC20.1-GUS and AtCDC20.2-GUS lines. Expressions of AtCDC20.1 in the primary root (A), leaf primordium (B) and in young stem segment (C) was identical to that of AtCDC20.2. Expression of AtCDC20.1 in the flower bud (C), anthers (D), pollen grains (E) and developing seeds (G) was specific while AtCDC20.2 was expressed in the sepals and style (F), but not in the silique (H). Blue color marks the β-glucuronidase activity of the GUS reporter gene. (I) to (P) Expression patterns of the AtCDC20.1-GFP or AtCDC20.2-GFP lines. DIC image of the root meristem at lower (I) and higher (K) magnifications and that of the leaf primordium (M). AtCDC20.1-GFP expression in the root meristem at lower (J), and higher (L) magnifications, in the leaf primordium (N) and in the flower bud (O). The expression pattern of AtCDC20.2-GFP was overlapping with that of AtCDC20.1-GFP with the exception of the flower buds where the AtCDC20.2-GFP signal was at the background level (P). Bright green color reflects the GFP fluorescence, the size of the root meristem is indicated with two-way red arrows, yellow arrow marks the leaf primordium and the red one the flower buds. Scale bars = 200 µm.