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. 2011 Jun 8;6(6):e20768. doi: 10.1371/journal.pone.0020768

Table 1. List of oligo nucleotides used for various purposes in the present study.

Oligonucleotides Sequence
Cathepsin L Sense: 5′-GACTCTGAGGAATCCTATCCA-3′ 180 bp amplicon
Antisense: 5′-AAGGACTCATGACCTGCATCAA-3′
Cathepsin B Sense: 5′-TGTAATGGTGGCTATCCTGCT-3′ 180 bp amplicon
Antisense: 5′-AGGCTCACAGATCTTGCTACA-3′
Cystatin-C Sense: 5′-CAGCAACGACATGTACCACAG-3′ 180 bp amplicon
Antisense: 5′-TTCCTTTTCAGATGTGGCTGGT-3′
EMSA oligos C/EBP.1 WT Sense: 5′- TTTTTATGTAATAA -3′
C/EBP. 1 WT Antisense: 5′- TTATTACATAAAAA -3′
C/EBP.2 WT Sense: 5′-TAATTACATAAATT-3′
C/EBP. 2 WT Antisense: 5-′AATTTATGTAATTA-3′
C/EBP.1 MT Sense: 5′- TTTTTtctTAATAA -3′
C/EBP.1 MT Antisense: 5′- TTATTAagaAAAAA -3′
C/EBP.2 MT Sense: 5′- TAATTAagaAAATT-3′
C/EBP.1 MT Antisense: 5-′AATTTATtctATTA-3′
ChIP primers Flanking the region of cathepsin L promoter containing C/EBP alpha motifs Sense: CF 5′-TGGGGTAAAGGCAGAGGTAA-3′ 198 bp amplicon of mRNA
Antisense: CR 5′-TGGAGAAATGTTGTAAGAGGAAA-3′
C/EBPα siRNA 1 sequences cloned in pSilencer 1.U6 siRNA expression vector having Apa I and Eco RI flanking sites Sense1: 5′-TCTAGTATTTAGGATAACTTCAAGAGAGTTATCCTAAATACTA G AGTTTTTG-3′ Target region 1378–1392 of mRNA
Antisense 1: 5′-AATTCAAAAACTCTAGTATTTAGGATAA CTCTCTTGAAGTTATCCTAAATACTAGAGGGCC-3 Apa I and Eco RI adaptor sequences have been shown in bold face
C/EBPα siRNA 1 sequences cloned in pSilencer 1.U6 siRNA expression vector having Apa I and Eco RI flanking sites Sense 2: 5′-CGGTTGTTCCCCTAGTTCTATTCAAGAGATA GAACTAGGGGAACAACCTTTTTG-3′ Target region 1621–1639 of mRNA
Antisense 2: 5′-AATTCAAAAAGGTTGTTCCCCTAGTTCT ATCTCTTGAATAGAACTAGGGGAACAACCGGGCC-3′ Apa I and Eco RI adaptor sequences have been shown in bold face
Scrambled siRNA sequences cloned in pSilencer 1.U6 siRNA expression vector having Apa I and Eco RI flanking sites Sense: 5′-CTTAAGTCATAGCTATTGAATTCAAGAGAT TCAATAGCTATGACTTAAG-3′
Antisense: 5′-AATTCTTAAGTCATAGCTATTGAATCTC TTGAATTCAATAGCTATGACTTAAGGGCC-3′ Apa I and Eco RI adaptor sequences have been shown in bold face
Oligos used for deletion construct of CTSL promoter pRB8 Sense: 5-GGTACCAAGGTTATGGGGTAAAGGCA GAGG-3′ KpnI site (Bold face)
pRB7.5 Sense: 5′CGGGGTACCGAAATGACAAAAGTT TAGAATG-3′
Antisense: 5′-AATTAAGCTTTCCTGCTGCGGTCGTAGC TGC-3′ HindIII site (Bold face)
Oligos used for site directed mutagenesis pRB9 Sense: 5′-AAGGTACCAGCCTGAACAACAGAGCC A-3′
C/EBP 1 Mut Antisense: 5′-GTCATTTCATTTTTTATTAagaAAAA ACATTCATTG-3
C/EBP 1 Mut Sense: 5′-CTACAATGAATGTTTTTtctTAATAAAA AAT GAAATG-3′
C/EBP 2 mut Sense: 5′-ATCCTTAATTAagaAAATTAAT ATCTACA-3′
C/EBP 2 mut Antisense: 5′-GTAGATATTAATTTtctTAATTAAGGAT-3′
RSBI Antisense: 5′-AATTAAGCTTTCCTGCTGCGGTCGTAGCTGC-3′

Mutated nucleotides are shown in lower case and bold face.