Table 1. List of oligo nucleotides used for various purposes in the present study.
Oligonucleotides | Sequence | |
Cathepsin L | Sense: 5′-GACTCTGAGGAATCCTATCCA-3′ | 180 bp amplicon |
Antisense: 5′-AAGGACTCATGACCTGCATCAA-3′ | ||
Cathepsin B | Sense: 5′-TGTAATGGTGGCTATCCTGCT-3′ | 180 bp amplicon |
Antisense: 5′-AGGCTCACAGATCTTGCTACA-3′ | ||
Cystatin-C | Sense: 5′-CAGCAACGACATGTACCACAG-3′ | 180 bp amplicon |
Antisense: 5′-TTCCTTTTCAGATGTGGCTGGT-3′ | ||
EMSA oligos | C/EBP.1 WT Sense: 5′- TTTTTATGTAATAA -3′ | |
C/EBP. 1 WT Antisense: 5′- TTATTACATAAAAA -3′ | ||
C/EBP.2 WT Sense: 5′-TAATTACATAAATT-3′ | ||
C/EBP. 2 WT Antisense: 5-′AATTTATGTAATTA-3′ | ||
C/EBP.1 MT Sense: 5′- TTTTTtctTAATAA -3′ | ||
C/EBP.1 MT Antisense: 5′- TTATTAagaAAAAA -3′ | ||
C/EBP.2 MT Sense: 5′- TAATTAagaAAATT-3′ | ||
C/EBP.1 MT Antisense: 5-′AATTTATtctATTA-3′ | ||
ChIP primers Flanking the region of cathepsin L promoter containing C/EBP alpha motifs | Sense: CF 5′-TGGGGTAAAGGCAGAGGTAA-3′ | 198 bp amplicon of mRNA |
Antisense: CR 5′-TGGAGAAATGTTGTAAGAGGAAA-3′ | ||
C/EBPα siRNA 1 sequences cloned in pSilencer 1.U6 siRNA expression vector having Apa I and Eco RI flanking sites | Sense1: 5′-TCTAGTATTTAGGATAACTTCAAGAGAGTTATCCTAAATACTA G AGTTTTTG-3′ | Target region 1378–1392 of mRNA |
Antisense 1: 5′-AATTCAAAAACTCTAGTATTTAGGATAA CTCTCTTGAAGTTATCCTAAATACTAGAGGGCC-3 | Apa I and Eco RI adaptor sequences have been shown in bold face | |
C/EBPα siRNA 1 sequences cloned in pSilencer 1.U6 siRNA expression vector having Apa I and Eco RI flanking sites | Sense 2: 5′-CGGTTGTTCCCCTAGTTCTATTCAAGAGATA GAACTAGGGGAACAACCTTTTTG-3′ | Target region 1621–1639 of mRNA |
Antisense 2: 5′-AATTCAAAAAGGTTGTTCCCCTAGTTCT ATCTCTTGAATAGAACTAGGGGAACAACCGGGCC-3′ | Apa I and Eco RI adaptor sequences have been shown in bold face | |
Scrambled siRNA sequences cloned in pSilencer 1.U6 siRNA expression vector having Apa I and Eco RI flanking sites | Sense: 5′-CTTAAGTCATAGCTATTGAATTCAAGAGAT TCAATAGCTATGACTTAAG-3′ | |
Antisense: 5′-AATTCTTAAGTCATAGCTATTGAATCTC TTGAATTCAATAGCTATGACTTAAGGGCC-3′ | Apa I and Eco RI adaptor sequences have been shown in bold face | |
Oligos used for deletion construct of CTSL promoter | pRB8 Sense: 5-GGTACCAAGGTTATGGGGTAAAGGCA GAGG-3′ | KpnI site (Bold face) |
pRB7.5 Sense: 5′CGGGGTACCGAAATGACAAAAGTT TAGAATG-3′ | ||
Antisense: 5′-AATTAAGCTTTCCTGCTGCGGTCGTAGC TGC-3′ | HindIII site (Bold face) | |
Oligos used for site directed mutagenesis | pRB9 Sense: 5′-AAGGTACCAGCCTGAACAACAGAGCC A-3′ | |
C/EBP 1 Mut Antisense: 5′-GTCATTTCATTTTTTATTAagaAAAA ACATTCATTG-3 | ||
C/EBP 1 Mut Sense: 5′-CTACAATGAATGTTTTTtctTAATAAAA AAT GAAATG-3′ | ||
C/EBP 2 mut Sense: 5′-ATCCTTAATTAagaAAATTAAT ATCTACA-3′ | ||
C/EBP 2 mut Antisense: 5′-GTAGATATTAATTTtctTAATTAAGGAT-3′ | ||
RSBI Antisense: 5′-AATTAAGCTTTCCTGCTGCGGTCGTAGCTGC-3′ |
Mutated nucleotides are shown in lower case and bold face.