Figure 8. Measurements of nitroreductase activities in YHΔacg using GSNO (A) or nitrofuran (B) as a substrate.

Presence of nitroreductase activities was measured by the reduction of the substrate using the bacterial cell lysates at 24 hours for GSNO and 48 hours for nitrofuran. The cell lysate extracted from E. coli was added as a positive control. The denatured cell lystaes which were produced by boiling the cell lysates at 100°C for 10 minutes were included as the negative control. The experiments were repeated twice with the similar results.