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The structure of PB-I in the wild type, esp1, esp3 and Esp4 as viewed by double indirect immunofluorescence microscopy. Endosperm sections from the wild type and the three mutant lines were labeled with antibodies against CysR10 and CysP13, followed by labeling with secondary antibodies conjugated with rhodamine and FITC, respectively. Note that the small pinpoint red dots in (A–E) are non-specific background, which is observed occasionally in immunofluorescence microscopy. Bars: 10 μm.
