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. 2011 Apr 26;52(6):1003–1016. doi: 10.1093/pcp/pcr053

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© The Author 2011. Published by Oxford University Press on behalf of Japanese Society of Plant Physiologists.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.5), which permits unrestricted non-commercial use distribution, and reproduction in any medium, provided the original work is properly cited.

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Fig. 8 — Down-regulation of CysR10 by RNAi. (A) SDS–PAGE analysis of the prolamin/glutelin fraction extracted from mature seed. Lane 1, wild-type, Kinmaze; lane 2, esp3 mutant derived from Kinmaze; lane 3, CysR10-repressed transformant derived from Yukihikari; lane 4, wild-type, Yukihikari. (B) Immunoblot analysis of the prolamin/glutelin fraction with anti-prolamin antibodies. (C) Confocal laser scanning microscopy of rhodamine-stained wild-type endosperm cells. (D) Confocal laser scanning microscopy of rhodamine-stained endosperm cells from the CysR10-repressed transformant. Bars in (C, D): 5 μm.