Fig. 3.

ISA3–GFP is localized in the amyloplast stroma in the rice endosperm. (A) Immunoblotting with anti-ISA3 antibody on seed proteins extracted from developing seeds of wild-type and transgenic plants expressing ISA3-GFP under the control of the rice ISA3 promoter. (B) Co-expression of ISA3-GFP under the control of the ISA3 promoter and tpCherry under the control of the α-globulin promoter in the developing endosperm. ISA3–GFP is localized in the amyloplast stroma and small particles indicated by arrowheads. The fluorescence signals of ISA3–GFP (left panel) and tpCherry (middle panel) are converted to green and magenta, respectively, and the images are merged (right panel). Bars = 5 μm.