. 2011 May 6;52(6):1068–1082. doi: 10.1093/pcp/pcr058

Table 1.

Starch content and amylase activities in the leaf blades

Plant	Starch content (% FW)		α-Amylase activity (U FW g⁻¹)^a	β-Amylase activity (U FW g⁻¹)^b
	End of the day	End of the night
Wild type	1.98 ± 0.04	0.05 ± 0.01	0.96 ± 0.04	1.02 ± 0.01
isa3	2.09 ± 0.06	1.23 ± 0.03*	1.10 ± 0.01*	1.16 ± 0.01*

The values are the average for three replications (mean ± SE).

Significant differences from the wild type are marked with an asterisk (P < 0.01, t-test).

^a One unit of α-amylase activity, Ceralpha Unit (Megazyme), is defined as the amount of enzyme, in the presence of excess α-glucosidase and glucoamylase, required to release 1 μmol of p-nitrophenol from BPNPG7 in 1 min under the defined assay conditions.

^b One unit of β-amylase activity, Betamyl Unit (Megazyme), is defined as the amount of enzyme required, in the presence of excess β-glucosidase, to release 1 μmol of p-nitrophenol from PNPG5 in 1 min under the defined assay conditions.