. 2011 May 6;52(6):1068–1082. doi: 10.1093/pcp/pcr058

Table 2.

Starch content in endosperm and bran, and amylase activities

Plant	Weight of 100 grains (g)	Endosperm						Bran
		Starch content (% FW)^a	Amylose content (% total starch)	α-Amylase activity (U FW g⁻¹)^b		β-Amylase activity (U FW g⁻¹)^c		Starch content (% FW)^a
				5 DAF	10 DAF	5 DAF	10 DPA
Wild type	2.89 ± 0.01	80.9 ± 0.1	23.0 ± 0.1	0.40 ± 0.01	0.28 ± 0.01	0.28 ± 0.02	0.24 ± 0.01	6.6 ± 0.1
isa3	2.89 ± 0.02	79.1 ± 0.3*	22.9 ± 0.1	0.52 ± 0.01*	0.36 ± 0.01*	0.31 ± 0.01	0.32 ± 0.02	19.5 ± 0.2*

The values are the average for three replications (mean ± SE).

Significant differences from the wild type are marked with an asterisk (P < 0.01, t-test).

^a Mature isa3 and wild-type grains were polished with a rice polisher (Kett Electric Laboratory) until 10% polishing was achieved. The starch contents of the polished endosperm and the bran were measured.

^b One unit of α-amylase activity, Ceralpha Unit (Megazyme), is as in Table 1.

^c One unit of β-amylase activity, Betamyl Unit (Megazyme), is as in Table 1.