Fig. 5.

Hierarchical cluster analysis of differentially expressed miRNAs during MPC differentiation. Cluster analysis was performed for 139 differentially expressed miRNAs after data adjustment (log transformation, median center, and normalization). The color codes of red, black, and blue represent high, average, and low expression levels, respectively. Five clusters were identified (A–E). For each cluster, a detailed view of expression changes is presented for individual miRNAs within that cluster; the average value of miRNA expression within each cluster is presented as a red line. Data were derived from 4 different MPC primary cultures with the cell differentiation assays initiated at passage 3.