Fig. 6.

Effects of inhibition of miR-682 in cultured, proliferating MPC. MPC were subcultured after the third passage and maintained in MPC growth medium on type I collagen-coated dishes. Transfection with locked nucleic acid-enhanced antisense or scramble oligonucleotide was accomplished at subculture, and cells were allowed to grow for 2 days. A: miR-682 expression levels were measured by quantitative real-time PCR. B: total cell number was determined manually. C: the distribution of cells in different phases of the cell cycle was established by flow cytometry. Data represent means ± SD of 3 biological repeats. *P ≤ 0.01 compared with the scramble oligonucleotide.