Table 3.
Recovery of WT and variant sodium channels in Q1077del background under both baseline pH and internal acidosis (pH 6.7) conditions
| Recovery at Baseline pH |
Recovery at pH 6.7 |
|||||||
|---|---|---|---|---|---|---|---|---|
| Samples | τf, ms | τs, ms | As, % | n | τf, ms | τs, ms | As, % | n |
| WT | 1.8 ± 0.3 | 45.1 ± 6.2 | 25.3 ± 1.7 | 7 | 3.2 ± 0.2 | 71.2 ± 6.2 | 24.4 ± 1.4 | 18 |
| S1103Y | 2.2 ± 0.3 | 45.0 ± 8.8 | 22.0 ± 1.7 | 8 | 2.4 ± 0.2* | 56.1 ± 5.2* | 24.4 ± 1.5 | 12 |
| R680H | 1.7 ± 0.2 | 34.2 ± 3.0 | 24.7 ± 2.4 | 9 | 3.7 ± 0.7 | 79.6 ± 9.8 | 23.2 ± 1.9 | 8 |
| S1103Y+R680H | 1.8 ± 0.2 | 42.1 ± 4.1 | 23.6 ± 1.7 | 10 | 2.5 ± 0.2* | 50.4 ± 3.8* | 25.7 ± 1.6 | 16 |
| S1103Y/R680H | 1.7 ± 0.2 | 37.7 ± 3.0 | 23.8 ± 1.1 | 14 | 2.5 ± 0.1* | 53.2 ± 3.3* | 25.5 ± 1.9 | 16 |
Values are means ± SE for n experiments. Time course of recovery from inactivation was analyzed by fitting data with a 2-exponential (exp) function: normalized INa (t) = Af[1 − exp(−t/τf)] + As[1 − exp(−t/τS)], where t is time, Af and As are fractional amplitudes of fast and slow components, respectively, and τf and τs are fast and slow time constants, respectively.
*
P < 0.05 vs. WT (1-way ANOVA followed by a Tukey test).