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. Author manuscript; available in PMC: 2011 Jun 9.
Published in final edited form as: J Immunol. 2010 Apr 16;184(10):5485–5492. doi: 10.4049/jimmunol.0902219

Figure 1. Flow cytometric analysis of CD31 expression on blood leukocytes.

Figure 1

(A) Schematic representation of membrane-bound CD31 and of the monoclonal antibodies used to detect the CD31 domains 2 (WM59) and 6 (MBC 78.2) on peripheral blood leukocytes. (B) Representative example of flow cytometric analysis of human peripheral blood cells from a healthy donor. CD8+ and CD4+ subpopulations were gated within CD3+ cells and were further analyzed for the expression of CD45RA. The proportion of cells lacking domain 2 was dramatically increased in memory (CD45RA−) cells compared to naïve (CD45RA+) cells. All leukocytes were positive for CD31 domain 6. Isotype controls of WM59 and MBC 78.2 antibodies are shown in the insets. (C) Fresh peripheral blood-derived resting CD4+ T-cells (top) lost their CD31 domain 2 upon TCR-activation (bottom).