Figure 3.
Analysis of tRNASer variants with substitutions in the acceptor stem and T-stem. Strains with different tRNASer variants were grown overnight, serially diluted, plated on YPD, and incubated for 2–3 d at 25°C, 30°C, 33°C, 35°C, and 37°C, and growth was examined and compared with the predicted stability of the acceptor and T-stems (Reuter and Mathews 2010). The highest temperature at which growth was observed is listed, and, in each case, healthy growth is observed at the next lower temperature. For the tRNASer(CGA) C51:U63 variant, growth was evaluated only by replica plating. (+++) Healthy growth; (+) poor but distinct growth; (green) healthy growth at 37°C; (yellow) intermediate growth; (red) lethal; (N/A) met22-Δ derivative strain not made, since healthy growth was observed without suppression; (N/D) strain not made.