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Destabilization of the acceptor stem of tRNASer(CGA) and tRNASer(IGA) results in a temperature-sensitive growth defect that is rescued by a met22-Δ mutation. Strains were grown and sampled as in Figure 1B. (A) Substitution of A7:U66 by G7:U66 in tRNASer(CGA). (B) Substitution of C6:G67 by U6:G67 in tRNASer(IGA/CGA).
