Figure 6.

Fidelity of LifeTrace and Phred quality scores. Quality scores associated with all basecalls aligned to the true sequence were binned into intervals of width Δ(q-score) = 2. Semi-logarithmic plot shows observed error rate in each bin as a function of quality score associated with that bin for the dye-primer and dye-terminator MegaBACE chromatogram set analyzed. Only substitution and insertion errors are considered here as deletion errors are captured by the newly introduced gap-quality score (see Fig. 3), and a deleted base itself does not have a quality as it does not exist. Ideal refers to the ideal line of.