Table 1.
Lysosomes per cell (mean value) | Lysosomes in nonphagocitosing cell (mean value) | % of phagocitosing cells | Bacteria per cell (mean value) | % of bacteria not in endosomes | % of Mθ̣ morphologically activateda | |
---|---|---|---|---|---|---|
WT | 16.2 | 17.5 | 62 | 2.1 | 7 | 76 |
Pml−/− | 7.8 | 7.4 | 25 | 2 | 25 | 25 |
Note: Total number of Mθ scored from 7 fields (3,000x-4,400x) in one electron microscopy section: wild-type (WT) = 21; Pml −/− = 24.
Macrophages were considered activated when showing extensive ruffling of the plasma membrane and a reduced nucleus/cytoplasm ratio.