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. Author manuscript; available in PMC: 2012 Jun 15.
Published in final edited form as: J Immunol. 2011 May 18;186(12):6990–6998. doi: 10.4049/jimmunol.1100396

Fig. 5. CF macrophages have reduced TLR4 protein degradation and translocation to the lysosomal compartment.

Fig. 5

(A) Representative western blot of TLR4 from WT and CF macrophages treated with protein synthesis inhibitor and challenged with LPS. (B) Mean ± SEM relative quantification normalized to beta-actin with time zero is set at 1 for 3 independent experiments. Statistical analysis was performed by two-sample t-tests; (C) IF for TLR4 (green), LAMP-1 (red) in WT (top) and CF (bottom) macrophages treated with LPS for 45 minutes; nuclei were stained with DAPI; (D) representative western blot (left) and relative quantification normalized to b-actin (right) for Rab7 in WT and CF macrophages untreated or challenged with LPS as indicated.