Fig. 1.

Hepatocytes were isolated from mice and exposed to room air, 3% oxygen, or 1% oxygen for either 15 minutes or 1 hour. HIF-1α was then detected in nuclear extracts by western blot. (A) Representative western blot of an n=3. (B) Intensity of the HIF-1α band was quantified, and the fold change relative to room air was determined. Data are expressed as means ± SEM; n = 3, where each n represents hepatocytes isolated from a different mouse.