Figure 3. Increased Accumulation of Bone Marrow-Derived Macrophages in Injured Vessels from PTEN iKO Mice.
(A) Immunofluorescence staining for GFP on 3-w injured femoral arteries from WT and PTEN iKO mice. Total numbers of GFP(+) cells in the arterial intima, media, and adventitia were counted separately and the data reported in the graphs. (B) Immunofluorescence staining for GFP and BrdU on 3-w injured femoral arteries from WT and PTEN iKO mice. GFP(+);BrdU(+) cells in the arterial intima, media, and adventitia were counted separately and the data reported in the graphs. Arrows = representative GFP(+);BrdU(+) cells; arrowheads = representative GFP(−);BrdU(+) cells. *Different from WT; n=8. N=Neointima; M=Media; A=Adventitia.